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Western blot analysis of extracts of various cell lines, using PIK3R3 antibody (13-965).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded mouse brain using PIK3R3 antibody (13-965) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using PIK3R3 antibody (13-965) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of SH-SY-5Y cells using PIK3R3 antibody (13-965).
Immunofluorescence analysis of PC-3M cells using PIK3R3 antibody (13-965).
Western blot analysis of extracts of various cell lines, using PIK3R3 antibody (13-965).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

PIK3R3 Antibody [4F10A1]

CATALOG NUMBER: 13-965

Clonality:
Monoclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein of human PIK3R3
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:1000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
MCF7
Positive Control 2:
Jurkat
Positive Control 3:
Mouse testis
Predicted Molecular Weight:
Observed: 54kDa
Purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
PIK3R3
Additional Names:
PIK3R3, DKFZp686P05226, FLJ41892, p55-GAMMA, p55
Ncbi Gene Id Number:
8503
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Phosphatidylinositol 3-kinase (PI3K) phosphorylates phosphatidylinositol and similar compounds, which then serve as second messengers in growth signaling pathways. PI3K is composed of a catalytic and a regulatory subunit. The protein encoded by this gene represents a regulatory subunit of PI3K. The encoded protein contains two SH2 domains through which it binds activated protein tyrosine kinases to regulate their activity.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-965

List Size:
50 uL

List Price:

$350.00

Datasheet
Shipping Info

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