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Western blot analysis in WiDr cell line lysates (35ug/lane).
PIK3R5 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in WiDr cell line lysates (35ug/lane).

PIK3R5 Antibody

CATALOG NUMBER: 55-906

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This PIK3R5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 763-792 amino acids from the C-terminal region of human PIK3R5.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
97 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PIK3R5
Additional Names:
Phosphoinositide 3-kinase regulatory subunit 5, PI3-kinase regulatory subunit 5, PI3-kinase p101 subunit, Phosphatidylinositol 4,5-bisphosphate 3-kinase regulatory subunit, PtdIns-3-kinase regulatory subunit, Protein FOAP-2, PtdIns-3-kinase p101, p101-PI3K, PIK3R5
Protein Accession Number:
Q8WYR1
Protein Gi Number:
74716480
Ncbi Gene Id Number:
23533
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Receptor-regulated class I phosphoinositide 3-kinases(PI3Ks) phosphorylate the membrane lipid phosphatidylinositol4,5-bisphosphate (PtdIns(4,5)P2) to PtdIns(3,4,5)P3, which in turnrecruits and activates cytosolic effectors involved inproliferation, survival, or chemotaxis. PIK3R5 is a PI3K regulatorysubunit (Brock et al., 2003 [PubMed 12507995]).
Background Reference 1:
Rose, J. Phd, et al. Mol. Med. (2010) In press :
Background Reference 2:
Segat, L., et al. Vaccine 28(10):2201-2206(2010)
Background Reference 3:
Yerges, L.M., et al. J. Bone Miner. Res. 24(12):2039-2049(2009)
Background Reference 4:
Johnson, C., et al. Oncogene 26(49):7049-7057(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-906

List Size:
400 ul

List Price:

$495.00

Datasheet
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