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Western blot analysis of PIK4CB Antibody in Hela cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PIK4CB Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of PIK4CB Antibody in Hela cell line lysates (35ug/lane)

PIK4CB Antibody

CATALOG NUMBER: 62-557

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Zebrafish, Mouse, Rat
Immunogen:
This PIK4CB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 519-548 amino acids from the Central region of human PIK4CB.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
91 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PI4KB
Additional Names:
Phosphatidylinositol 4-kinase beta, PI4K-beta, PI4Kbeta, PtdIns 4-kinase beta, NPIK, PI4K92, PI4KB, PIK4CB
Protein Accession Number:
Q9UBF8
Protein Gi Number:
38372507
Ncbi Gene Id Number:
5298
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PIK4CB phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate (PIP). It may regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation.
Background Reference 1:
Jeganathan,S., et.al., Mol. Cell. Biol. 28 (14), 4549-4561 (2008) Pizarro-Cerda,J., et.al., Cell. Microbiol.(10), 2381-2390 (2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-557

List Size:
400 ul

List Price:

$495.00

Datasheet
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