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Western blot analysis of PIKE in mouse brain cell lysates with PIKE antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.
Immunohistochemistry of PIKE in mouse brain tissue with PIKE antibody at 10 μg/mL.
Western blot analysis of PIKE in mouse brain cell lysates with PIKE antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.
Trial Size

PIKE Antibody

CATALOG NUMBER: 3321

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (86%)
Immunogen:
PIKE antibody was raised against a peptide corresponding to 15 amino acids near the C-terminus of human PIKE.
The immunogen is located within the last 50 amino acids of PIKE.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
PIKE antibody can be used for detection of PIKE by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
Specificity:
Anti-PIKE will detect both PIKE-L and PIKE-A isoforms.
Positive Control 1:
Cat. No. 1403 - Mouse Brain Tissue Lysate
Purification:
PIKE Antibody is Ion exchange chromatography purified.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PIKE Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
PIKE antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
AGAP2
Additional Names:
PIKE Antibody: PIKE, GGAP2, CENTG1, KIAA0167, Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2, Centaurin-gamma-1, AGAP-2
Protein Accession Number:
AAM97540
Protein Gi Number:
25989575
Ncbi Gene Id Number:
116986
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PIKE Antibody: Phosphoinositide 3 kinase enhancer (PIKE) is a recently identified nuclear GTPase that interacts with nuclear phosphoinositide 3-kinase (PI3 kinase) to stimulate its lipid kinase activity. PIKE exists in multiple isoforms; a shorter C-terminal isoform (PIKE-A) has also been identified as centaurin gamma 1. The longest isoform (PIKE-L) has been shown to bind to the adaptor protein Homer and thereby link to metabotropic glutamate receptors, leading to activation of PI3 kinase activity and prevention of neuronal apoptosis. Overexpression of PIKE-A enhances Akt activity and promotes cancer cell invasion, whereas decreased expression of PIKE-A via dominant negative expression of PIKE-A or PIKE-A knockdown inhibits these processes. In many human cancers, expression of PIKE-A is enhanced, leading to increased Akt activity and preventing apoptosis.
Background Reference 1:
Ye K, Hurt KJ, Wu FY, et al. Pike. A nuclear gtpase that enhances PI3kinase activity and is regulated by protein 4.1N. Cell 2000; 103:919-30.
Background Reference 2:
Jackson TR, Kearns BG, and Thiebert AB. Cytohesins and centaurins: mediators of PI3-kinase-regulated Arf signaling. Trends Biochem. Sci. 2000; 25:489-95.
Background Reference 3:
Rong R, Ahn JY, Huang H, et al. PI3 kinase enhancer-Homer complex couples mGluRI to PI3 kinase, preventing neuronal apoptosis. Nat. Neurosci. 2003; 6:1153-61.
Background Reference 4:
Ahn J-Y, Rong R, Kroll TG, et al. PIKE (Phosphotidylinositol 3-kinase enhancer)-A GTPase stimulates Akt activity and mediates cellular invasion. J. Biol. Chem. 2004; 279:16441-51.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3321

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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