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46-997 (0.3ug/ml) staining of Human Amygdala lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-997 (3.75ug/ml) staining of paraffin embedded Human Thyroid. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-997 (3.75ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-997 (0.3ug/ml) staining of Human Amygdala lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

PIKE Antibody

CATALOG NUMBER: 46-997

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-QASLDSIREAVINSQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 100kDa band observed in Human Brain (Amygdala and Cerebral Cortex) lysates (calculated MW of 90.5kDa according to NP_055585.1). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Thyroid and Kidney. Recommended concentration: 3.75ug/ml.
Specificity:
This antibody is expected to recognize isoform PIKE-S (NP_055585.1).
Positive Control 1:
Cat. No. XBL-10131 - Human Amygdala Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
AGAP2
Additional Names:
CENTG1, centaurin, gamma 1, AGAP2, FLJ16430, GGAP2, KIAA0167, PIKE, Arf GAP with GTP-binding protein-like, ANK repeat and PH domains 2, GTP-binding and GTPase activating protein 2, centaurin gamma1, phosphoinositide 3-kinase enhancer
Protein Accession Number:
NP_055585.1
Protein Gi Number:
7661962
Ncbi Gene Id Number:
116986
Background Reference 1:
Liu Z, Jang SW, Liu X, Cheng D, Peng J, Yepes M, Li XJ, Matthews S, Watts C, Asano M, Hara-Nishimura I, Luo HR, Ye K. Neuroprotective actions of PIKE-L by inhibition of SET proteolytic degradation by asparagine endopeptidase. Mol. Cell 2008 Mar 29 (6): 665-78.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-997

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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