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Western blot analysis of extracts from HUVEC cells treated with PMA using Pim-1 (Phospho-Tyr309) Antibody. The lane on the right is treated with the antigen-specific peptide.
Western blot analysis of extracts from HUVEC cells treated with PMA using Pim-1 (Phospho-Tyr309) Antibody. The lane on the right is treated with the antigen-specific peptide.

Pim-1 (phospho Tyr309) Antibody

CATALOG NUMBER: 79-800

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Pim-1 (phospho Tyr309) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 309 (A-A-V (p)-W-S) derived from Human Pim-1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
The antibody detects endogenous levels of Pim-1 only when phosphorylated at tyrosine 309.
Predicted Molecular Weight:
45 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
PIM1
Additional Names:
PIM-1, Proto-oncogene serine/threonine-protein kinase pim-1, EC 2.7.11.1
Protein Accession Number:
NP_001230115.1
Protein Gi Number:
83305339
Ncbi Gene Id Number:
5292
Background:
Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation and thus providing a selective advantage in tumorigenesis. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression and by phosphorylation and inhibition of proapoptotic proteins (BAD, MAP3K5, FOXO3). Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase of transcriptional activity. The stabilization of MYC exerted by PIM1 might explain partly the strong synergism between these two oncogenes in tumorigenesis.
Background Reference 1:
Selten G., Cell 46:603-611(1986).
Background Reference 2:
Saris C.J., EMBO J. 10:655-664(1991).
Background Reference 3:
Maita H., Eur. J. Biochem. 267:5168-5178(2000).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-800

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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