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Western blot analysis of extracts of various cell lines, using PIM1 antibody (13-906).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human spleen using PIM1 antibody (13-906) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human mammary cancer using PIM1 antibody (13-906) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using PIM1 antibody (13-906) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using PIM1 antibody (13-906) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using PIM1 antibody (13-906) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of SW480 cells using PIM1 antibody (13-906).
Immunofluorescence analysis of BT-20 cells using PIM1 antibody (13-906).
Immunofluorescence analysis of HepG2 cells using PIM1 antibody (13-906).
Western blot analysis of extracts of various cell lines, using PIM1 antibody (13-906).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

PIM1 Antibody [G7B0]

CATALOG NUMBER: 13-906

Clonality:
Monoclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant protein of human PIM1
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:5000 - 1:10000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
BT-20
Positive Control 2:
HepG2
Positive Control 3:
NIH/3T3
Predicted Molecular Weight:
Observed: 33kDa
Purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
PIM1
Additional Names:
PIM1, Pim-1 kinase 44 kDa isoform, Proviral integration site 1, Oncogene PIM1, PIM, Pim-1, Pim-1 oncogene
Ncbi Gene Id Number:
5292
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene belongs to the Ser/Thr protein kinase family, and PIM subfamily. This gene is expressed primarily in B-lymphoid and myeloid cell lines, and is overexpressed in hematopoietic malignancies and in prostate cancer. It plays a role in signal transduction in blood cells, contributing to both cell proliferation and survival, and thus provides a selective advantage in tumorigenesis. Both the human and orthologous mouse genes have been reported to encode two isoforms (with preferential cellular localization) resulting from the use of alternative in-frame translation initiation codons, the upstream non-AUG (CUG) and downstream AUG codons (PMIDs:16186805, 1825810).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-906

List Size:
50 uL

List Price:

$335.00

Out of stock

Datasheet
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