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HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human PIM2 with C-terminal MYC tag probed with 43-115 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with 43-115 (1mg/ml) in Lane B. Primary i
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human PIM2 with C-terminal MYC tag probed with 43-115 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with 43-115 (1mg/ml) in Lane B. Primary i

PIM2 Antibody

CATALOG NUMBER: 43-115

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-PPGGKDREAFEAE
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:In transfected HEK293 transiently expressing Human PIM2 (myc and DYKDDDDK tagged), a band of approx. 38kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-myc antibody. Recommended concentration, 1-3ug/ml.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PIM2
Additional Names:
PIM2, pim-2 oncogene, HGNC:8987, proto-oncogene Pim-2 (serine threonine kinase), serine/threonine protein kinase pim-2
Protein Accession Number:
NP_006866.2
Protein Gi Number:
42821112
Ncbi Gene Id Number:
11040
Background Reference 1:
Hammerman PS, Fox CJ, Cinalli RM, Xu A, Wagner JD, Lindsten T, Thompson CB. Lymphocyte transformation by Pim-2 is dependent on nuclear factor-kappaB activation.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-115

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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