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HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human PIM2 with C-terminal MYC tag probed with 43-337 (0.1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with 43-337 (0.1mg/ml) in Lane B. Detec
43-337 (5ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human PIM2 with C-terminal MYC tag probed with 43-337 (0.1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with 43-337 (0.1mg/ml) in Lane B. Detec

PIM2 Antibody

CATALOG NUMBER: 43-337

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-QTPAEDVPLNPSK
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:In transfected HEK293 transiently expressing Human PIM2 (myc and DYKDDDDK tagged), a band of approx. 38kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-myc antibody. Recommended concentration: 0.5-1ug/ml. Primary incubations were for 1 hour.Immunohistochemistry:Paraffin embedded Human Testis. Recommended concentration: 5ug/ml.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PIM2
Additional Names:
PIM2, pim-2 oncogene, pim-2h, proto-oncogene Pim-2 (serine threonine kinase), serine/threonine protein kinase pim-2, serine/threonine-protein kinase pim-2
Protein Accession Number:
NP_006866.2
Protein Gi Number:
42821112
Ncbi Gene Id Number:
11040
Background Reference 1:
Morishita D, Katayama R, Sekimizu K, Tsuruo T, Fujita N, Pim kinases promote cell cycle progression by phosphorylating and down-regulating p27Kip1 at the transcriptional and posttranscriptional levels.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-337

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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