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Antibody is used in Western blot to detect PIM3 in mouse kidney tissue lysate.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PIM3 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Formalin-fixed and paraffin-embedded human Spleen tissue reacted with PIM3 antibody , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
Antibody is used in Western blot to detect PIM3 in mouse kidney tissue lysate.

PIM3 Antibody

CATALOG NUMBER: 62-703

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This PIM3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 259-288 amino acids from the C-terminal region of human PIM3.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
36 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PIM3
Additional Names:
Serine/threonine-protein kinase pim-3, PIM3
Protein Accession Number:
Q86V86
Protein Gi Number:
215274221
Ncbi Gene Id Number:
415116
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PIM3 is a memberof the Ser/Thr protein kinase family that may be involved in cell cycle progression and anti-apoptosis process. This protein has been implicated in proliferation of human hepatoma cell lines. PIM3 is widely expressed, with the exception of no expression observed in colon, thymus, and small intestine. PIM3 is expressed in human hepatoma cell lines but not in normal liver tissues.
Background Reference 1:
Mikkers, H., et al., Mol. Cell. Biol. 24(13):6104-6115 (2004). Eichmann, A., et al., Oncogene 19(9):1215-1224 (2000).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-703

List Size:
400 ul

List Price:

$495.00

Datasheet
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