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46-189 (0.2ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-189 (0.2ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

PIN1 Antibody

CATALOG NUMBER: 46-189

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Rat
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse, Dog
Immunogen:
The immunogen for this antibody is: C-KHSQSRRPSSWRQEKITR
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 17kDa band observed in Rat Brain lysates (calculated MW of 18.2kDa according to Human NP_006212.1 and 18.2kDa according to Mouse NP_075860). Recommended concentration: 0.2-0.6ug/ml. Preliminary testing was unsuccessful on Mouse Brain for this particular batch.
Positive Control 1:
Cat. No. 1463 - Rat Brain Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PIN1
Additional Names:
PIN1, protein (peptidylprolyl cis/trans isomerase) NIMA-interacting 1 , DOD, UBL5 , peptidyl-prolyl cis/trans isomerase, NIMA-interacting, prolyl isomerase, protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting 1
Protein Accession Number:
NP_006212.1
Protein Gi Number:
5453898
Ncbi Gene Id Number:
5300
Background Reference 1:
Pastorino L, Sun A, Lu PJ, Zhou XZ, Balastik M, Finn G, Wulf G, Lim J, Li SH, Li X, Xia W, Nicholson LK, Lu KP. The prolyl isomerase Pin1 regulates amyloid precursor protein processing and amyloid-beta production. Nature. 2006 Mar 23;440(7083):528-34.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-189

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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