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Western blot analysis of PINK1 in A431 cell lysate with PINK1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of PINK1 in mouse brain tissue with PINK1 Antibody at 5 μg/mL.
Immunofluorescence of PINK1 in mouse brain tissue with PINK1 Antibody at 20 μg/mL. <br><br>Green: PINK1 antibody (7859) <br> Red: Phylloidin staining<br> Blue: DAPI staining
Western blot analysis of PINK1 in (A) mouse and (B) rat spleen tissue lysate with PINK1 antibody at 1 μg/mL.
Western blot analysis of PINK1 in A431 cell lysate with PINK1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Trial Size

PINK1 Antibody

CATALOG NUMBER: 7859

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
PINK1 antibody was raised against a 16 amino acid peptide near the amino terminus of human PINK1.
The immunogen is located within amino acids 120 - 170 of PINK1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
PINK1 antibody can be used for detection of PINK1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human, mouse, and rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
PINK1 antibody is human, mouse and rat reactive. At least two isoforms are known to exist; this antibody will only detect the longer isoform. PINK1 antibody will detect the cleaved and uncleaved form of PINK1.
Positive Control 1:
Cat. No. 1202 - A431 Cell Lysate
Positive Control 2:
Cat. No. 1406 - Mouse Spleen Tissue Lysate
Positive Control 3:
Cat. No. 1466 - Rat Spleen Tissue Lysate
Predicted Molecular Weight:
Predicted: 55 kDa
Observed: 53 kDa
Purification:
PINK1 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PINK1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
PINK1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
PINK1
Additional Names:
PINK1 Antibody: BRPK, PARK6BRPK
Protein Accession Number:
NP_115785
Protein Gi Number:
14165272
Ncbi Gene Id Number:
65018
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The PTEN-induced putative kinase 1 (PINK1) is a serine/threonine protein kinase that localizes to mitochondria and is thought to protect cells from stress-induced mitochondrial dysfunction (reviewed in 1). PINK1 recruits the E3 ubiquitin ligase Parkin to mitochondria to initiate mitophagy, an autophagic process that clears damaged mitochondria within a cell (2). PINK1 is cleaved by the mitochondrial protease PARL (3). Mutations in this gene cause one form of autosomal recessive early-onset Parkinson disease (4).
Background Reference 1:
Matsuda S, Kitagishi Y, and Kobayashi M. Function and characteristics of PINK1 in mitochondria. Oxid. Med. Cell. Longev. 2013;601587.
Background Reference 2:
Corti O, Lesage S, and Brice A. What genetics tells us about the causes and mechanism of Parkinson's disease. Physiol. Rev. 2011; 91:1161-218.
Background Reference 3:
Deas E, Plun-Favreau H, and Gandhi S, et al. PINK1 cleavage at position A103 by the mitochondrial protease PARL. Hum. Mol. Genet. 2011; 20:867-79.
Background Reference 4:
Valente EM, Abou-Sleiman PM, Caputo V, et al. Hereditary early-onset Parkinson's disease caused by mutations in PINK1. Science 2004; 304:1158-60.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7859

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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