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Western blot analysis of extracts of various cell lines, using PIP4K2A antibody (22-946) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat kidney using PIP4K2A antibody (22-946) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using PIP4K2A antibody (22-946) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using PIP4K2A antibody (22-946).
Western blot analysis of extracts of various cell lines, using PIP4K2A antibody (22-946) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 90s.

PIP4K2A Antibody

CATALOG NUMBER: 22-946

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 120-350 of human PIP4K2A (NP_005019.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
Jurkat
Positive Control 2:
MCF7
Positive Control 3:
Mouse brain
Positive Control 4:
Mouse spinal cord
Positive Control 5:
Rat brain
Predicted Molecular Weight:
Observed: 46kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
PIP4K2A
Additional Names:
PIP4K2A, PI(5)P 4-kinase type II alpha, PIP5KII-alpha, PIP5KIII, PIPK, PtdIns(4)P-5-kinase B isoform, PtdIns(4)P-5-kinase C isoform, PI5P4KA, PIP4KII-alpha, PIP5K2, PIP5KIIalpha, PIP5K2A, PIP5KIIA
Ncbi Gene Id Number:
5305
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Phosphatidylinositol-5,4-bisphosphate, the precursor to second messengers of the phosphoinositide signal transduction pathways, is thought to be involved in the regulation of secretion, cell proliferation, differentiation, and motility. The protein encoded by this gene is one of a family of enzymes capable of catalyzing the phosphorylation of phosphatidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring to form phosphatidylinositol-5,4-bisphosphate. The amino acid sequence of this enzyme does not show homology to other kinases, but the recombinant protein does exhibit kinase activity. This gene is a member of the phosphatidylinositol-5-phosphate 4-kinase family.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-946

List Size:
100 uL

List Price:

$436.00

Datasheet
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