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46-193 (0.3ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-193 (5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-193 (5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-193 (0.3ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Pirin Antibody

CATALOG NUMBER: 46-193

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse
Immunogen:
The immunogen for this antibody is: GSSKKVTLSVLSREC
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 33kDa band observed in Human Heart lysate (calculated MW of 32.1kDa according to NP_003653.1). Recommended concentration: 0.3 - 1.0 ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Liver and Spleen. Recommended concentration: 5ug/ml.
Specificity:
Variants (NP_003653.1; NP_001018119.1) encode the same protein
Positive Control 1:
Cat. No. 1301 - Human Heart Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PIR
Additional Names:
PIR, pirin, pirin (iron-binding nuclear protein)
Protein Accession Number:
NP_003653.1
Protein Gi Number:
4505823
Ncbi Gene Id Number:
8544
Background Reference 1:
Wendler WM, Kremmer E, Forster R, Winnacker EL. Identification of pirin, a novel highly conserved nuclear protein. J Biol Chem. 1997 Mar 28;272(13):8482-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-193

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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