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Western blot analysis of PISD Antibody in mouse cerebellum tissue and mouse NIH-3T3 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain reacted with PISD Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of PISD Antibody in mouse cerebellum tissue and mouse NIH-3T3 cell line lysates (35ug/lane)

PISD Antibody

CATALOG NUMBER: 63-660

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This PISD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 223-250 amino acids from the Central region of human PISD.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
47 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PISD
Additional Names:
Phosphatidylserine decarboxylase proenzyme, Phosphatidylserine decarboxylase alpha chain, Phosphatidylserine decarboxylase beta chain, PISD
Protein Accession Number:
Q9UG56
Protein Gi Number:
311033492
Ncbi Gene Id Number:
23761
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Phosphatidylserine decarboxylases catalyze the formation of phosphatidylethanolamine (PE) by decarboxylation of phosphatidylserine (PS). Type I PSDs, such as PISD, are targeted to the inner mitochondrial membrane by an N-terminal targeting sequence. PISD also contains a conserved LGST motif that functions as an autocatalytic cleavage site where the proenzyme is split into mature alpha and beta subunits
Background Reference 1:
Simpson,J.C., et.al., EMBO Rep.(3), 287-292 (2000)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-660

List Size:
400 ul

List Price:

$495.00

Datasheet
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