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Western blot analysis of PIST in PC-3 cell lysate with PIST antibody at (A) 1, (B) 2 and (C) 4 μg/mL.
Immunohistochemistry of PIST in rat colon tissue with PIST antibody at 1 μg/mL.
Immunofluorescence of PIST in Rat Colon cells with PIST antibody at 20 μg/mL.
Western blot analysis of PIST in PC-3 cell lysate with PIST antibody at (A) 1, (B) 2 and (C) 4 μg/mL.
Trial Size

PIST Antibody

CATALOG NUMBER: 3633

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
PIST antibody was raised against a 17 amino acid synthetic peptide from near the center of human PIST.
The immunogen is located within amino acids 240 - 290 of PIST.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
PIST antibody can be used for the detection of PIST by Western blot at 1 - 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 1 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1216 - PC-3 Cell Lysate
Positive Control 2:
Cat. No. 1472 - Rat Colon Tissue Lysate
Purification:
PIST Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PIST Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
PIST antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GOPC
Additional Names:
PIST Antibody: CAL, FIG, PIST, GOPC1, dJ94G16.2, CAL, Golgi-associated PDZ and coiled-coil motif-containing protein, CFTR-associated ligand
Protein Accession Number:
AAG00572
Protein Gi Number:
9837431
Ncbi Gene Id Number:
57120
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PIST Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components and is negatively regulated by TOR (Target of rapamycin). PIST, a PDZ-containing protein, was discovered in a yeast two-hybrid system as a binding partner to Beclin-1, a Bcl-2-interacting protein homologous to the yeast autophagy gene apg6. Experiments with mutant PIST proteins lacking the PDZ domain showed that PIST interaction with Beclin-1 through its coiled-coil domain can modulate Beclin-1 activity and suggest that PIST interactions with other proteins through its PDZ domain may regulate the activity of PIST and Beclin-1.
Background Reference 1:
Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene. 2004; 23:2891-906.
Background Reference 2:
Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis 1993; 14:2501-5.
Background Reference 3:
Kamada Y, Funakoshi T, Shintani T, et al. Tor-mediated induction of autophagy via Apg1 protein kinase complex. J. Cell. Biol. 2000; 150:1507-13.
Background Reference 4:
Yu Z, Horton A, Bravin M, et al. A novel protein complex linking the δ2 glutamate receptor and autophagy: implications for neurodegeneration in Lurcher mice. Neuron 2002; 35:921-33.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3633

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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