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46-194 (0.05ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-194 (0.05ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

PIST Antibody

CATALOG NUMBER: 46-194

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: C-LDDLHTLYHKKSY
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx. 55kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 50.5kDa according to NP_065132.1). Recommended concentration: 0.05-0.2ug/ml.Immunofluorescence: This product has been successfully used on HeLa cells.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_065132.1; NP_001017408.1).
Positive Control 1:
Cat. No. 1366 - Human Frontal Lobe Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
GOPC
Additional Names:
PIST, GOPC, CAL, PDZ/coiled-coil domain binding partner for the rho-family GTPase TC10, fused in glioblastoma, CFTR-associated ligand, Golgi associated PDZ and coiled-coil motif containing protein, golgi associated PDZ and coiled-coil motif containing, CAL, FIG, GOPC1, dJ94G16.2, dJ94G16.2 PIST
Protein Accession Number:
NP_065132.1,NP_001017408.1
Protein Gi Number:
9966877
Ncbi Gene Id Number:
57120
Background Reference 1:
Neudauer CL, Joberty G, Macara IG. PIST: a novel PDZ/coiled-coil domain binding partner for the rho-family GTPase TC10. Biochem Biophys Res Commun. 2001 Jan 19;280(2):541-7.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-194

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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