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Western blot analysis of various lysates using Pit1/POU1F1 Rabbit mAb at 1:8000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 10s.
Western blot analysis of various lysates using Pit1/POU1F1 Rabbit mAb at 1:8000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Negative control (NC): Rat liver | Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded Rat pituitary gland tissue using Pit1/POU1F1 Rabbit mAb at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse pituitary gland tissue using Pit1/POU1F1 Rabbit mAb at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Confocal imaging of paraffin-embedded Rat pituitary tissue using Pit1/POU1F1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
Confocal imaging of paraffin-embedded Mouse pituitary tissue using Pit1/POU1F1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
Immunoprecipitation of Pit1/POU1F1 from 300 µg extracts of Mouse pituitary tissue was performed using 2 µg of Pit1/POU1F1 Rabbit mAb. Rabbit Control IgG was used to precipitate the Control IgG sample. IP samples were eluted with. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using Pit1/POU1F1 Rabbit mAb at a dilution of 1:9000.
Western blot analysis of various lysates using Pit1/POU1F1 Rabbit mAb at 1:8000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 10s.

Pit1/POU1F1 Rabbit Recombinant Antibody (83-594)

CATALOG NUMBER: 83-594

Clonality:
Monoclonal
Tested Applications:
ELISA, IF-P, IHC-P, IP, WB
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF-P, IHC-P, IP, WB
Application Note:
WB,1:4000 - 1:16000, IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells, IF-P,1:200 - 1:800, IHC-P,1:1000 - 1:4000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Predicted Molecular Weight:
33kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
POU1F1
Additional Names:
PIT1; CPHD1; GHF-1; Pit-1; POU1F1a
Protein Accession Number:
P28069
Ncbi Gene Id Number:
5449
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the POU family of transcription factors that regulate mammalian development. The protein regulates expression of several genes involved in pituitary development and hormone expression. Mutations in this genes result in combined pituitary hormone deficiency. Multiple transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

83-594

List Size:
100 uL

List Price:

$477.00

Datasheet
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