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Western blot analysis of PITX1 antibody in NCI-H460 cell line lysates (35ug/lane)
PITX1 Antibody (RB18913) IHC analysis in formalin fixed and paraffin embedded human Lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of PITX1 using rabbit polyclonal PITX1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PITX1 gene.
Western blot analysis of PITX1 antibody in NCI-H460 cell line lysates (35ug/lane)

PITX1 Antibody

CATALOG NUMBER: 62-833

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Chicken, Mouse, Rat, Xenopus, Zebrafish, Drosophila, C. Elegans
Immunogen:
This PITX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 123-150 amino acids from the Central region of human PITX1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
34 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PITX1
Additional Names:
Pituitary homeobox 1, Hindlimb-expressed homeobox protein backfoot, Homeobox protein PITX1, Paired-like homeodomain transcription factor 1, PITX1, BFT, PTX1
Protein Accession Number:
P78337
Protein Gi Number:
108935922
Ncbi Gene Id Number:
5307
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PITX1 is a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. Members of this family are involved in organ development and left-right asymmetry. This protein acts as a transcriptional regulator involved in basal and hormone-regulated activity of prolactin.
Background Reference 1:
Grimminger,P., Ling,F.C. Anticancer Res. 29 (4), 1281-1286 (2009)
Background Reference 2:
Gurnett,C.A., Alaee,F. Am. J. Hum. Genet. 83 (5), 616-622 (2008)
Background Reference 3:
Philippi,A., Tores,F. BMC Med. Genet. 8, 74 (2007)
Background Reference 4:
Shang,J., Li,X. Genomics 40 (1), 108-113 (1997)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-833

List Size:
400 ul

List Price:

$495.00

Datasheet
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