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Antibody used in IHC on Human Intestine.
Antibody used in WB on Human HepG2 at 2.5 ug/ml.
Antibody used in IHC on Human Intestine.

PITX2 Antibody

CATALOG NUMBER: 28-320

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PITX2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
PITX2 antibody can be used for detection of PITX2 by ELISA at 1:62500. PITX2 antibody can be used for detection of PITX2 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
36 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store PITX2 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
PITX2
Additional Names:
PITX2, RS, RGS, ARP1, Brx1, IDG2, IGDS, IHG2, PTX2, RIEG, IGDS2, IRID2, Otlx2, RIEG1
Protein Accession Number:
NP_000316
Protein Gi Number:
21361183
Ncbi Gene Id Number:
5308
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The PITX2 gene encodes a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. This protein acts as a transcription factor and regulates procollagen lysyl hydroxylase gene expression. Mutations in PITX2 are associated with Axenfeld-Rieger syndrome (ARS), iridogoniodysgenesis syndrome (IGDS), and sporadic cases of Peters anomaly. This protein is involved in the development of the eye, tooth and abdominal organs. It also acts as a transcriptional regulator involved in basal and hormone-regulated activity of prolactin.
Background Reference 1:
Martin, D.M., et al., (2004) Dev. Biol. 267 (1), 93-108.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

28-320

List Size:
100 ul

List Price:

$473.00

Datasheet
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