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Western blot analysis of PIWI-L2 in HepG2 cell lysate with PIWI-L1 antibody at 1 μg/mL.
Immunocytochemistry of PIWI-L2 in HepG2 cells with PIWI-L2 antibody at 2.5 μg/ml.
Immunofluorescence of PIWI-L2 in HepG2 cells with PIWI-L2 antibody at 5 μg/ml.
Western blot analysis of PIWI-L2 in HepG2 cell lysate with PIWI-L1 antibody at 1 μg/mL.
Trial Size

PIWI-L2 Antibody

CATALOG NUMBER: 6569

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (87%), Chicken: (75%)
Immunogen:
PIWI-L2 antibody was raised against a 17 amino acid synthetic peptide near the center of human PIWI-L2.
The immunogen is located within amino acids 410 - 460 of PIWI-L2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
PIWI-L2 antibody can be used for detection of PIWI-L2 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
At least three isoforms of PIWI-L2 are known to exist; this antibody will only detect the two longest isoforms. PIWI-L2 antibody is predicted to not cross-react with other PIWI protein family members.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Positive Control 2:
Cat. No. 17-011 - Hep G2 Cell Slide
Purification:
PIWI-L2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PIWI-L2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
PIWI-L2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PIWIL2
Additional Names:
PIWI-L2 Antibody: CT80, HILI, mili, PIWIL1L, Piwi-like protein 2, CT80
Protein Accession Number:
NP_00129193
Protein Gi Number:
24431985
Ncbi Gene Id Number:
55124
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PIWI-L2 Antibody: PIWI-L2 is a member of the PIWI subfamily of Argonaute proteins, evolutionarily conserved proteins containing both PAZ and Piwi motifs that are crucial for the biogenesis and function of small non-coding RNAs and play important roles in stem cell self-renewal, RNA silencing, and translational regulation in diverse organisms. PIWI-L2 has been implicated in multiple roles including stem cell self-renewal and is highly expressed in many breast cancers. In these tumors, PIWI-L2 silences the expression of STAT3, a regulator of Bcl-XL and cyclin D1, allowing the proliferation and survival of breast cancer stem cells. Recent studies have shown that overexpression of PIWI-like proteins are potential biomarkers for colon and other cancers.
Background Reference 1:
Kuramochi-Miyagawa S, Kimura T, Yomogida K, et al. Two mouse piwi-related genes: miwi and mili. Mech. Dev. 108:121-33
Background Reference 2:
Thomson T and Lin H. The biogenesis and function PIWI proteins and piRNAs: progress and prospect. Annu. Rev. Cell Dev. Biol. 2009; 25:355-76.
Background Reference 3:
Lee JH, Jung C, Javadian-Elyaderani P, et al. Pathways of proliferation and antiapoptosis driven in breast cancer stem cells by stem cell protein Piwil2. Cancer Res. 2010; 70:4569-79.
Background Reference 4:
Li L, Yu C, Gao H, et al. Argonaute proteins: potential biomarkers for human colon cancer. BMC Cancer 2010; 10:38

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6569

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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