Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot analysis of PIWI-L3 in 3T3 cell lysate with PIWI-L3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunofluorescence of PIWI-L3 in 3T3 cells with PIWI-L3 antibody at 20 μg/mL.
Western blot analysis of PIWI-L3 in 3T3 cell lysate with PIWI-L3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

PIWI-L3 Antibody

CATALOG NUMBER: 6033

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
PIWI-L3 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human PIWI-L3.
The immunogen is located within amino acids 130 - 180 of PIWI-L3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
PIWI-L3 antibody can be used for detection of PIWI-L3 by Western blot at 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Positive Control 2:
Cat. No. 17-201 - 3T3/BALB Cell Slide
Purification:
PIWI-L3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PIWI-L3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
PIWI-L3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PIWIL3
Additional Names:
PIWI-L3 Antibody: HIWI3, Piwi-like protein 3
Protein Accession Number:
NP_001008496
Protein Gi Number:
153792298
Ncbi Gene Id Number:
440822
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PIWI-L3 Antibody: PIWI-L3 is a member of the PIWI subfamily of Argonaute proteins, evolutionarily conserved proteins containing both PAZ and Piwi motifs that are crucial for the biogenesis and function of small non-coding RNAs and play important roles in stem cell self-renewal, RNA silencing, and translational regulation in diverse organisms. Recent studies have shown that overexpression of PIWI-L3 (as well as other PIWI-like proteins) are potential biomarkers for astrocytic glioma, meningioma, and other cancers.
Background Reference 1:
Sasaki T, Shiohama A, Minoshima S, et al. Identification of eight members of the Argonaute family in the human genome small star, filled. Genomics2003; 323-30.
Background Reference 2:
Thomson T and Lin H. The biogenesis and function PIWI proteins and piRNAs: progress and prospect. Annu. Rev. Cell Dev. Biol.2009; 25:355-76.
Background Reference 3:
Wang XL, Chen XM, Gao HJ, et al. Preparation and distribution of polyclonal antibodies against human PIWIL3 protein in tumor tissues. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2008; 24:714-6.
Background Reference 4:
Li L, Yu C, Gao H, et al. Argonaute proteins: potential biomarkers for human colon cancer. BMC Cancer2010; 10:38.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6033

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369