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PJA1 Antibody

CATALOG NUMBER: 43-019

Clonality:
Polyclonal
Tested Applications:
ELISA
Host Species:
Goat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse
Immunogen:
The immunogen for this antibody is: C-HSTSRWRDTANDNE
Conjugate:
Unconjugated
Tested Applications:
ELISA
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Preliminary experiments gave an approx 30kDa band in Human Brain and Mouse Brain lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 50.4kDa according to NP_071763.2. The 30kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Specificity:
This antibody is expected to recognize all reported isoforms (NP_660095.1; NP_001027568.1; NP_071763.2).
Predicted Molecular Weight:
Approx 30 kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PJA1
Additional Names:
PJA1, praja ring finger 1, PRAJA1, RNF70, E3 ubiquitin-protein ligase Praja-1, OTTHUMP00000023457, OTTHUMP00000023458, OTTHUMP00000158771, RING finger protein 70, praja 1
Protein Accession Number:
NP_660095.1,NP_001027568.1,NP_071763.2
Protein Gi Number:
41281725
Ncbi Gene Id Number:
64219

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-019

List Size:
0.1 mg

List Price:

$391.00

Datasheet
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