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Western blot analysis of extracts from COLO205 cells using PKC alpha (Phospho-Tyr658) Antibody. The lane on the right is treated with the antigen-specific peptide.
Western blot analysis of extracts from COLO205 cells using PKC alpha (Phospho-Tyr658) Antibody. The lane on the right is treated with the antigen-specific peptide.

PKC alpha (phospho Tyr658) Antibody

CATALOG NUMBER: 79-806

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
PKC alpha (phospho Tyr658) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 658 (F-S-Y (p)-V-N) derived from Human PKC alpha.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
The antibody detects endogenous levels of PKC alpha only when phosphorylated at tyrosine 658.
Predicted Molecular Weight:
80 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
PRKCA
Additional Names:
PKC-alpha, KPCA, PKC III, PKC-A
Protein Accession Number:
NP_002728.1
Protein Gi Number:
317373571
Ncbi Gene Id Number:
5578
Background:
Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in positive and negative regulation of cell proliferation, apoptosis, differentiation, migration and adhesion, tumorigenesis, cardiac hypertrophy, angiogenesis, platelet function and inflammation, by directly phosphorylating targets such as RAF1, BCL2, CSPG4, TNNT2/CTNT, or activating signaling cascade involving MAPK1/3 (ERK1/2) and RAP1GAP. Involved in cell proliferation and cell growth arrest by positive and negative regulation of the cell cycle. Can promote cell growth by phosphorylating and activating RAF1, which mediates the activation of the MAPK/ERK signaling cascade, and/or by up-regulating CDKN1A, which facilitates active cyclin-dependent kinase (CDK) complex formation in glioma cells.
Background Reference 1:
Finkenzeller G., Nucleic Acids Res. 18:2183-2183(1990).
Background Reference 2:
McSwine-Kennick R.L., J. Biol. Chem. 266:15135-15143(1991).
Background Reference 3:
Gevaert K., Nat. Biotechnol. 21:566-569(2003)

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-806

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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