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Western blot analysis of PRKCI using rabbit polyclonal PKC iota Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKCI gene (Lane 2).
Western blot analysis of hPKC-iota-C181 in Hela cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot analysis of PRKCI using rabbit polyclonal PKC iota Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKCI gene (Lane 2).

PKC iota Antibody

CATALOG NUMBER: 62-596

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This PKC iota antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 176-207 amino acids from the N-terminal region of human PKC iota.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
68 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PRKCI
Additional Names:
Protein kinase C iota type, Atypical protein kinase C-lambda/iota, PRKC-lambda/iota, aPKC-lambda/iota, nPKC-iota, PRKCI, DXS1179E
Protein Accession Number:
P41743
Protein Gi Number:
239938658
Ncbi Gene Id Number:
5584
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PKC iota belongs to the protein kinase C (PKC) family of serine/threonine protein kinases. The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehydes-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis.
Background Reference 1:
Zhang, J., et al., J. Biol. Chem. 279(21):22118-22123 (2004).
Background Reference 2:
Roehrl, M.H., et al., J. Biomol. NMR 26(4):373-374 (2003).
Background Reference 3:
Tisdale, E.J., J. Biol. Chem. 278(52):52524-52530 (2003).
Background Reference 4:
Suzuki, A., et al., J. Biochem. 133(1):9-16 (2003).

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-596

List Size:
400 ul

List Price:

$495.00

Datasheet
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