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Western blot analysis of anti-PKC theta Pab in placenta lysate. PKC theta was detected using purified Pab.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot analysis of anti-PKC theta Pab in placenta lysate. PKC theta was detected using purified Pab.

PKC theta Antibody

CATALOG NUMBER: 62-601

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This PKC theta antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 318-348 amino acids from the C-terminal region of human PKC theta.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
82 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PRKCQ
Additional Names:
Protein kinase C theta type, nPKC-theta, PRKCQ, PRKCT
Protein Accession Number:
Q04759
Protein Gi Number:
20141582
Ncbi Gene Id Number:
5588
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. PKC theta is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors.
Background Reference 1:
Carrasco, S., et al., Mol. Biol. Cell 15(6):2932-2942 (2004).
Background Reference 2:
Sun, L., et al., Blood 103(3):948-954 (2004).
Background Reference 3:
Burchfield, J.G., et al., J. Biol. Chem. 279(18):18623-18632 (2004).
Background Reference 4:
Ishaq, M., et al., J. Biol. Chem. 278(41):39296-39302 (2003).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-601

List Size:
400 ul

List Price:

$495.00

Datasheet
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