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Western blot analysis of anti-PKCzeta Pab in placenta lysate
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with PKC zeta antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of anti-PKCzeta Pab in placenta lysate

PKC zeta Antibody

CATALOG NUMBER: 62-602

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This PKC zeta antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 166-197 amino acids from the N-terminal region of human PKC zeta.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
68 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PRKCZ
Additional Names:
Protein kinase C zeta type, nPKC-zeta, PRKCZ, PKC2
Protein Accession Number:
Q05513
Protein Gi Number:
68067736
Ncbi Gene Id Number:
5590
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a constitutive kinase activity which is independent of calcium, and PKC activators, phosphatidylserine and diacylglycerol. Furthermore, it is insensitive to PKC inhibitors and cannot be activated by phorbol ester. The structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC.
Background Reference 1:
Li, Y.F., et al., World J. Gastroenterol. 9(9):2078-2082 (2003).
Background Reference 2:
Minami, T., et al., J. Biol. Chem. 278(9):6976-6984 (2003).
Background Reference 3:
Beeson, M., et al., Diabetes 52(8):1926-1934 (2003).
Background Reference 4:
Laudanna, C., et al., Lab. Invest. 83(8):1155-1163 (2003).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-602

List Size:
400 ul

List Price:

$495.00

Datasheet
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