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Western blot analysis of extracts from HeLa cells treated with Heatshock using Phospho-PKCB (Ser661) Antibody. The lane on the right is treated with the antigen-specific peptide.
Western blot analysis of extracts from HeLa cells treated with Heatshock using Phospho-PKCB (Ser661) Antibody. The lane on the right is treated with the antigen-specific peptide.

PKCB (phospho Ser661) Antibody

CATALOG NUMBER: 79-957

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
PKCB (phospho Ser661) antibody was raised against a peptide sequence around phosphorylation site of serine 661 (G-F-S (p)-Y-T) derived from Human PKCB.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
The antibody detects endogenous level of PKCB only when phosphorylated at serine 661.
Predicted Molecular Weight:
77 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
PRKCB
Additional Names:
PIM-1, EC 2.7.11.1
Protein Accession Number:
NP_002729.2
Protein Gi Number:
20127450
Ncbi Gene Id Number:
5579
Background:
Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase involved in various cellular processes such as regulation of the B-cell receptor (BCR) signalosome, oxidative stress-induced apoptosis, androgen receptor-dependent transcription regulation, insulin signaling and endothelial cells proliferation. Plays a key role in B-cell activation by regulating BCR-induced NF-kappa-B activation. Phosphorylation induces CARD11/CARMA1 association with lipid rafts and recruitment of the BCL10-MALT1 complex as well as MAP3K7/TAK1, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1.
Background Reference 1:
Zhu M, Chen J, Jiang H, Miao C (2013) Cardiovasc Diabetol 12, 13
Background Reference 2:
Leonard TA, et al. (2011) Cell 144, 55-66
Background Reference 3:
Qiao M, et al. (2010) Mol Cell 38, 512-23

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-957

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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