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Western blot analysis of lysed extracts from PC12 cells using PKCδ (Ab-645).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PKCδ (Ab-645).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using PKCδ (Ab-645).
Western blot analysis of lysed extracts from PC12 cells using PKCδ (Ab-645).

PKCdelta (Ab 645) Antibody

CATALOG NUMBER: 79-401

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
PKCdelta (Ab-645) antibody was raised against a peptide sequence around aa. 643~647 (R-L-S-Y-S) derived from Human PKCδ.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Specificity:
This antibody detects endogenous level of total PKCδ protein.
Predicted Molecular Weight:
78 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
PRKCD
Additional Names:
MAY1, PKCD, CVID9, nPKC-delta, G0S7
Protein Accession Number:
NP_006245.2
Protein Gi Number:
31377782
Ncbi Gene Id Number:
5580
Background:
This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol. which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. May play a role in antigen-dependent control of B-cell function. Phosphorylates MUC1 in the C-terminal and regulates the interaction between MUC1 and beta-catenin.
Background Reference 1:
Kei Sakamoto, et, al. (2003) Am J Physiol Endocrinol Metab, ; 285: E1081 - E1088. Ling Zhang, et, al. (2004) J. Biol. Chem ; 279: 28315 - 28319. Kristof Van Kolen et, al. (2006) FEBS J ; 273: 1843 - 1854. Martin Villalba, et, al. (2002) J. Cell Biol ; 157: 253.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-401

List Size:
0.1 mg

List Price:

$370.00

Datasheet
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