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Western blot analysis of lysed extracts from PC12 cells untreated or treated with PMA using PKCΘ (Phospho-Ser676).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PKCΘ (Phospho-Ser676).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using PKCΘ (Phospho-Ser676).
Western blot analysis of lysed extracts from PC12 cells untreated or treated with PMA using PKCΘ (Phospho-Ser676).

PKCtheta (phospho Ser676) Antibody

CATALOG NUMBER: 79-230

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
PKCtheta (Phospho-Ser676) antibody was raised against a peptide sequence around phosphorylation site of serine 676 (R-L-S (p) -F-A) derived from Human PKCΘ.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Specificity:
This antibody detects endogenous level of PKCΘ only when phosphorylated at serine 676.
Predicted Molecular Weight:
80 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
PRKCQ
Additional Names:
PRKCT, nPKC-theta, HSP90B, HSPC2, HSPCB, KPCT, PKC-theta, PKCQ, PRKCQ, PRKCT
Protein Accession Number:
NP_006248.1
Protein Gi Number:
5453976
Ncbi Gene Id Number:
5588
Background:
This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-κ-B in mature T lymphocytes. Required for interleukin-2 (IL2) production.
Background Reference 1:
Kristof Van Kolen, et al. (2006) FEBS J ; 273: 1843 - 1854. Martin Villalba, et al. (2002) J. Cell Biol ; 157: 253. Jie Zhang, et al. (2004) J. Biol. Chem ; 279: 22118 - 22123. Castro AF, et al. (1998) Am J Physiol Cell Physiol; 275: C113 - C119.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-230

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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