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Western blot analysis of extract from 293 cells and HeLa using PKCzeta (Ab-410).
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PKCzeta (Ab-410).
Western blot analysis of extract from 293 cells and HeLa using PKCzeta (Ab-410).

PKCzeta (Ab 410) Antibody

CATALOG NUMBER: 79-411

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
PKCzeta (Ab-410) antibody was raised against a peptide sequence around aa. 408~412 ( T-S-T-F-C) derived from PKCzeta .
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Specificity:
This antibody detects endogenous levels of total PKCzeta protein.
Predicted Molecular Weight:
78 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
PRKCZ
Additional Names:
PKC2, PKC-ZETA, GAD65, PKC2, PRKCZ
Protein Accession Number:
NP_002735.3
Protein Gi Number:
52486327
Ncbi Gene Id Number:
5590
Background:
This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization.
Background Reference 1:
Parmentier JH, et al. (2004) BMC Cell Biol; 5: 4. Castrillo A, et al. (2003) Mol Cell Biol; 23 (4): 1196-1208. Paramio JM, et al. ( 2001) Mol Cell Biol; 21 (21): 7449-7459. Braiman L, et al. (2001) Mol Cell Biol; 21 (22): 7852-7861.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-411

List Size:
0.1 mg

List Price:

$370.00

Datasheet
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