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Western blot analysis of extracts of various cell lines, using PKD2 antibody (19-107) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Western blot analysis of extracts of various cell lines, using PKD2 antibody (19-107) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

PKD2 Antibody

CATALOG NUMBER: 19-107

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 240-410 of human PKD2 (NP_000288.1).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:500 - 1:1000
Positive Control 1:
293T
Positive Control 2:
MCF7
Positive Control 3:
Mouse kidney
Predicted Molecular Weight:
Observed: 110kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
PKD2
Additional Names:
polycystwin, polycystin 2, PKD4, PC2, MGC138468, MGC138466, APKD2, polycystic kidney disease 2 (autosomal dominant), PKD2, Pc-2, TRPP2
Ncbi Gene Id Number:
5311
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the polycystin protein family. The encoded protein is a multi-pass membrane protein that functions as a calcium permeable cation channel, and is involved in calcium transport and calcium signaling in renal epithelial cells. This protein interacts with polycystin 1, and they may be partners in a common signaling cascade involved in tubular morphogenesis. Mutations in this gene are associated with autosomal dominant polycystic kidney disease type 2.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-107

List Size:
100 uL

List Price:

$436.00

Datasheet
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