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Western blot analysis of lysed extracts from K562 cells using PKR (Phospho-Thr446).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using PKR (Phospho-Thr446).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using PKR(Phospho-Thr446).
Western blot analysis of lysed extracts from K562 cells using PKR (Phospho-Thr446).

PKR (phospho Thr446) Antibody

CATALOG NUMBER: 79-227

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
PKR (Phospho-Thr446) antibody was raised against a peptide sequence around phosphorylation site of threonine 446 (K-R-T (p) -R-S) derived from Human PKR.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Specificity:
This antibody detects endogenous level of PKR only when phosphorylated at threonine 446.
Predicted Molecular Weight:
68 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
EIF2AK2
Additional Names:
PKR, PRKR, EIF2AK1, C1orf24, NIBAN, GIG39, ADRB2, E2AK2, EIF2AK2, EIF2aK
Protein Accession Number:
NP_001129123.1
Protein Gi Number:
208431827
Ncbi Gene Id Number:
5610
Background:
Following activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection.
Background Reference 1:
Abujiang Pataer, et, al. (2002) Cancer Res; 62: 2239. K. D. Ryman, et, al. (2005) J. Virol; 79: 1487 - 1499. Susana Guerra, et, al. (2006) J. Biol. Chem; 281: 18734 - 18745.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-227

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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