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Western blot analysis of PLA1A in human kidney tissue lysate with PLA1A antibody at (A) 1 and (B) 2 μg/mL.
Immunofluorescence of PLA1A in human kidney tissue with PLA1A antibody at 20 μg/mL.
Western blot analysis of PLA1A in human kidney tissue lysate with PLA1A antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

PLA1A Antibody

CATALOG NUMBER: 7059

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (75%), Bovine: (71%)
Immunogen:
PLA1A antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human PLA1A.
The immunogen is located within amino acids 380 - 430 of PLA1A.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
PLA1A antibody can be used for detection of PLA1A by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
At least three isoforms of PLA1A are known to exist; this antibody will detect all three isoforms.
Positive Control 1:
Cat. No. 1305 - Human Kidney Tissue Lysate
Positive Control 2:
Cat. No. 10-401 - Human Kidney Tissue Slide
Predicted Molecular Weight:
50 kDa
Purification:
PLA1A Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PLA1A Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
PLA1A antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PLA1A
Additional Names:
PLA1A Antibody: PSPLA1, PS-PLA1, NMD, PSPLA1, Phospholipase A1 member A, Phosphatidylserine-specific phospholipase A1
Protein Accession Number:
NP_056984
Protein Gi Number:
7706661
Ncbi Gene Id Number:
51365
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PLA1A Antibody: PLA1A is a phospholipase that hydrolyzes fatty acids at the sn-1 position of phosphatidylserine and 1-acyl-2-lysophosphatidylserine. This secreted protein hydrolyzes phosphatidylserine (PS) in liposomes and can also hydrolyze PS in apoptotic cells and activate platelets where the resulting 2-acyl-lysophosphatidylserine acts as a lipid mediator for mast cells, T cells, and neural cells, suggesting that a major function of PLA1A may be the production of lysophospholipid mediators. PLA1A is upregulated in rat peripheral blood cells bearing long-term surviving cardiac allograft. PLA1A is also expressed in human THP-1-derived macrophages and this expression is upregulated in cells treated with lipopolysaccharide, a TLR4 ligand. This upregulation is inhibited with corticosteroids, which are often used at high dosages to suppress chronic allograft rejection.
Background Reference 1:
Sato T, Aoki J, Nagai Y, et al. Serine phospholipid-specific phospholipase A that is secreted from activated platelets. A new member of the lipase family. J. Biol. Chem. 1997; 272:2192-8.
Background Reference 2:
Aoki J, Nagai Y, Hosono H, et al. Structure and function of phosphatidylserine-specific phospholipase A1. Biochim. Biophys. Acta 2002; 1582:26-32.
Background Reference 3:
Hosono H, Homma M, Ogasawa Y, et al. Expression of phosphatidylserine-specific phospholipase A(1) mRNA in human THP-1-derived macrophages. Cell Transplant. 2010; 19:759-64.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7059

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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