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Western blot analysis in A2058 cell line lysates (35ug/lane).
Western blot analysis of PLA2G12A using rabbit polyclonal PLA2G12A Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PLA2G12A gene.
Western blot analysis in A2058 cell line lysates (35ug/lane).

PLA2G12A Antibody

CATALOG NUMBER: 58-615

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This PLA2G12A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 55-83 amino acids from the Central region of human PLA2G12A.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
21 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PLA2G12A
Additional Names:
Group XIIA secretory phospholipase A2, GXII sPLA2, sPLA2-XII, Phosphatidylcholine 2-acylhydrolase 12A, PLA2G12A, PLA2G12
Protein Accession Number:
Q9BZM1
Protein Gi Number:
20143881
Ncbi Gene Id Number:
81579
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Secreted phospholipase A2 (sPLA2) enzymes liberatearachidonic acid from phospholipids for production of eicosanoidsand exert a variety of physiologic and pathologic effects. GroupXII sPLA2s, such as PLA2G12A, have relatively low specific activityand are structurally and functionally distinct from other sPLA2s(Gelb et al., 2000 [PubMed 11031251]).
Background Reference 1:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 2:
Kalsi, G., et al. Hum. Mol. Genet. 19(12):2497-2506(2010)
Background Reference 3:
Chen, W., et al. Proc. Natl. Acad. Sci. U.S.A. 107(16):7401-7406(2010)
Background Reference 4:
Hosgood, H.D. III, et al. Occup Environ Med 66(12):848-853(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-615

List Size:
400 ul

List Price:

$495.00

Datasheet
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