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Western blot analysis of extracts of THP-1 cells, using PLA2G4C antibody (23-158) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human tonsil using PLA2G4C antibody (23-158) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using PLA2G4C antibody (23-158) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of THP-1 cells, using PLA2G4C antibody (23-158) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

PLA2G4C Antibody

CATALOG NUMBER: 23-158

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-189 of human PLA2G4C (NP_003697.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
THP-1
Predicted Molecular Weight:
Observed: 80kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
PLA2G4C
Additional Names:
PLA2G4C, phospholipase A2, group IVC (cytosolic, calcium-independent), CPLA2-gamma, DKFZp586C0423, FLJ42247, FLJ44164, cytosolic phospholipase A2 gamma, phospholipase A2, cytosolic, calcium-independent, gamma, phospholipase A2, group IVC
Ncbi Gene Id Number:
8605
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a protein which is a member of the phospholipase A2 enzyme family which hydrolyzes glycerophospholipids to produce free fatty acids and lysophospholipids, both of which serve as precursors in the production of signaling molecules. The encoded protein has been shown to be a calcium-independent and membrane bound enzyme. Multiple transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-158

List Size:
100 uL

List Price:

$436.00

Datasheet
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