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Western blot analysis of PLAC3 in HeLa cell lysate with PLAC3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry of PLAC3 in HeLa cells with PLAC3 antibody at 10 μg/mL.
Immunofluorescence of PLAC3 in HeLa cells with PLAC3 antibody at 20 μg/mL.
Western blot analysis of PLAC3 in HeLa cell lysate with PLAC3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

PLAC3 Antibody

CATALOG NUMBER: 6019

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
PLAC3 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human PLAC3.
The immunogen is located within amino acids 450 - 500 of PLAC3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
PLAC3 antibody can be used for detection of PLAC3 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2:
Cat. No. 17-001 - HeLa Cell Slide
Purification:
PLAC3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PLAC3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
PLAC3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PAPPA2
Additional Names:
PLAC3 Antibody: PAPPE, PLAC3, PAPP-E, PAPP-A2, Pappalysin-2, Pregnancy-associated plasma protein A2
Protein Accession Number:
NP_064714
Protein Gi Number:
116174738
Ncbi Gene Id Number:
60676
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PLAC3 Antibody: PLAC3 (placenta-specific protein 3), also known as PAPP-A2, is a metalloproteinase that cleaves the insulin-like growth factor-binding protein (IGFBP)-5 in a IGF-independent fashion. It has been suggested that the cleavage of IGFBP-5 and to a lesser extent IGFBP-3 produced in the maternal decidua promotes feto-placental growth. Recent studies have shown elevated levels of PLAC3 in hypertensive disorders of pregnancy including preeclampsia and HELLP (hemolysis, elevated liver enzymes, and low platelets) syndrome.
Background Reference 1:
Overgaard MT, Boldt HB, Laursen LS, et al. Pregnancy-associated plasma protein-A2 (PAPP-A2), a novel insulin-like growth factor-binding protein-5 proteinase. J. Biol. Chem.2001; 276:1849-53.
Background Reference 2:
Wang J, Qiu Q, Haider M, et al. Expression of pregnancy-associated plasma protein A2 during pregnancy in human and mouse. J. Endocrin.2009; 202:337-45.
Background Reference 3:
Nishizawa H, Pryor-Koishi K, Suzuki M, et al. Increased levels of pregnancy-associated plasma protein-A2 in the serum of pre-eclamptic patients. Mol. Hum. Reprod.2008; 14:595-602.
Background Reference 4:
Buimer M, Keijser R, Jebbink JM, et al. Seven placental transcripts characterize HELLP syndrome. Placenta2008; 29:444-53.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6019

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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