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Western blot testing of human 1) U87, 2) HeLa and 3) A431 cell lysate with Plakophilin 1 antibody (clone 10B2). Expected molecular weight: 75-83 kDa.
SDS-PAGE Analysis of Purified, BSA-Free Plakophilin 1 Antibody (clone 10B2). Confirmation of Integrity and Purity of the Antibody.
Western blot testing of human 1) U87, 2) HeLa and 3) A431 cell lysate with Plakophilin 1 antibody (clone 10B2). Expected molecular weight: 75-83 kDa.

Plakophilin 1 Antibody [10B2]

CATALOG NUMBER: 33-749

Clonality:
Monoclonal
Tested Applications:
IF, WB
Host Species:
Mouse
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human, Mouse
Immunogen:
A N-terminal human recombinant protein fragment was used as the immunogen for the Plakophilin 1 antibody. Its epitope maps to between amino acids 1-27 of human PKP1.
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
WB: 0.5-1 ug/ml
IF: 1-2 ug/ml
Optimal dilution of the Plakophilin 1 antibody should be determined by the researcher.
Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG1, kappa
physical-state:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
PKP1
Ncbi Gene Id Number:
5317
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
Recognizes a protein of 75kDa, identified as Plakophilin 1 (PKP1). Plakophilins 1, 2, 3 and 4 (PKP1-4) influence development and participate in linking cadherins to cytoskeletal intermediate filaments. PKP1-4 contain arm-repeat (armadillo) domains and localize to nuclei and cell desmosomes (cell-cell junctions found in suprabasal layers of stratifying epithelia that undergo mechanical stress). PKP2 is important for desmosome assembly and is an essential morphogenic factor and architectural component of the heart.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-749

List Size:
100 ug

List Price:

$626.00

Datasheet
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