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Western blot analysis of extracts of NCI-H460 cells, using PLCB1 antibody (18-420) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat heart using PLCB1 antibody (18-420) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using PLCB1 antibody (18-420) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using PLCB1 antibody (18-420) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using PLCB1 antibody (18-420). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of NCI-H460 cells, using PLCB1 antibody (18-420) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

PLCB1 Antibody

CATALOG NUMBER: 18-420

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 917-1216 of human PLCB1 (NP_056007.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
NCI-H460
Predicted Molecular Weight:
Observed: 139kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
PLCB1
Additional Names:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1, PLC-154, Phosphoinositide phospholipase C-beta-1, Phospholipase C-I, PLC-I, Phospholipase C-beta-1, PLC-beta-1, PLCB1, KIAA0581
Ncbi Gene Id Number:
23236
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of many extracellular signals. This gene is activated by two G-protein alpha subunits, alpha-q and alpha-11. Two transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-420

List Size:
100 uL

List Price:

$436.00

Datasheet
Shipping Info

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