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Western blot analysis of PLCB1 Antibody in HL-60 cell line lysates (35ug/lane)
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of PLCB1 Antibody in HL-60 cell line lysates (35ug/lane)

PLCB1 Antibody

CATALOG NUMBER: 63-915

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse
Immunogen:
This PLCB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1148-1176 amino acids from the C-terminal region of human PLCB1.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
139 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PLCB1
Additional Names:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1, PLC-154, Phosphoinositide phospholipase C-beta-1, Phospholipase C-I, PLC-I, Phospholipase C-beta-1, PLC-beta-1, PLCB1, KIAA0581
Protein Accession Number:
Q9NQ66
Protein Gi Number:
12643814
Ncbi Gene Id Number:
23236
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of many extracellular signals. This gene is activated by two G-protein alpha subunits, alpha-q and alpha-11.
Background Reference 1:
Need, A.C., et al. Hum. Mol. Genet. 18(23):4650-4661(2009)
Background Reference 2:
Woodcock, E.A., et al. J. Mol. Cell. Cardiol. 47(5):676-683(2009)
Background Reference 3:
Zhang, Y., et al. J. Recept. Signal Transduct. Res. 29(1):52-62(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-915

List Size:
400 ul

List Price:

$495.00

Datasheet
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