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Western blot analysis of lysed extracts from 293 and 3T3 cells using PLCγ2 (Ab-753).
Immunofluorescence staining of methanol-fixed HeLa cells using PLCγ2 (Ab-753).
Western blot analysis of lysed extracts from 293 and 3T3 cells using PLCγ2 (Ab-753).

PLCgamma2 (Ab 753) Antibody

CATALOG NUMBER: 79-571

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
PLCgamma2 (Ab-753) antibody was raised against a peptide sequence around aa. 751~755 (S-L-Y-D-V) derived from Human PLCγ2.
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200
Specificity:
This antibody detects endogenous level of total PLCγ2 protein.
Predicted Molecular Weight:
150 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
PLCG2
Additional Names:
FCAS3, APLAID, PLC-IV, PLC-gamma-2, Phosphoinositide phospholipase C, Phospholipase C-gamma-2
Protein Accession Number:
NP_002652.2
Protein Gi Number:
117320537
Ncbi Gene Id Number:
5336
Background:
The production of the second messenger molecules diacylglycerol. (DAG) and inositol 1, 4, 5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.
Background Reference 1:
Kim YJ, et al. (2004) Mol Cell Biol 24: 9986-9999Humphries LA, et al. (2004) J Biol Chem 279 : 37651-37661Suzuki-Inoue K, et al. (2004) Biochem J 378 : 1023-1029Rodriguez R, et al. (2003) Biochem J 374 : 269-280

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-571

List Size:
0.1 mg

List Price:

$370.00

Datasheet
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