Western blot analysis in NCI-H460 cell line lysates (35ug/lane).

PLCL2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Confocal immunofluorescent analysis of PLCL2 Antibody with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis in NCI-H460 cell line lysates (35ug/lane).

PLCL2 Antibody

CATALOG NUMBER: 56-169

Clonality:

Polyclonal

Tested Applications:

Flow, IF, IHC-P, WB

Host Species:

Rabbit

Species Reactivity:

Human

Conjugate:

Unconjugated

Specifications

Host Species:

Rabbit

Species Reactivity:

Human

Immunogen:

This PLCL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1099-1126 amino acids from the C-terminal region of human PLCL2.

Conjugate:

Unconjugated

Tested Applications:

Flow, IF, IHC-P, WB

Application Note:

For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50

Predicted Molecular Weight:

126 kDa

Advanced Validation

Properties

Purification:

This antibody is purified through a protein A column, followed by peptide affinity purification.

Clonality:

Polyclonal

Isotype:

Rabbit Ig

Physical State:

Liquid

Buffer:

Supplied in PBS with 0.09% (W/V) sodium azide.

Concentration:

batch dependent

Storage Conditions:

Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Additional Info

Ncbi Official Symbol:

PLCL2

Additional Names:

Inactive phospholipase C-like protein 2, PLC-L(2), PLC-L2, Phospholipase C-L2, Phospholipase C-epsilon-2, PLC-epsilon-2, PLCL2, KIAA1092, PLCE2

Protein Accession Number:

Q9UPR0

Protein Gi Number:

148880116

Ncbi Gene Id Number:

23228

User Note:

Optimal dilutions for each application to be determined by the researcher.

Background

Background:

PLCL2 may play an role in the regulation of Ins(1,4,5)P3 around the endoplasmic reticulum (By similarity).

Background Reference 1:

Raychaudhuri, S., et al. Nat. Genet. 41(12):1313-1318(2009)

Background Reference 2:

Colland, F., et al. Genome Res. 14(7):1324-1332(2004)

Background Reference 3:

Nakayama, M., et al. Genome Res. 12(11):1773-1784(2002)

Background Reference 4:

Otsuki, M., et al. Biochem. Biophys. Res. Commun. 266(1):97-103(1999)

Citations

Set Contents

Disclaimer

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-169

List Size:

400 ul

List Price:

$495.00

Datasheet

Shipping Info

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$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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PLCL2 Antibody

Clonality:

Tested Applications:

Host Species:

Species Reactivity:

Conjugate:

Host Species:

Species Reactivity:

Immunogen:

Conjugate:

Tested Applications:

Application Note:

Predicted Molecular Weight:

Purification:

Clonality:

Isotype:

Physical State:

Buffer:

Concentration:

Storage Conditions:

Ncbi Official Symbol:

Additional Names:

Protein Accession Number:

Protein Gi Number:

Ncbi Gene Id Number:

User Note:

Background:

Background Reference 1:

Background Reference 2:

Background Reference 3:

Background Reference 4:

Disclaimer:

List Size:

Shipping Info

Customize your order

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