PLD1 (phospho Thr147) Antibody

CATALOG NUMBER: 79-927

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
PLD1 (phospho Thr147) antibody was raised against a peptide sequence around phosphorylation site of threonine 147 (R-H-T (p)-F-R) derived from Human PLD1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
The antibody detects endogenous levels of PLD1 only when phosphorylated at threonine 147.
Predicted Molecular Weight:
120 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
PLD1
Additional Names:
PLD 1, hPLD1, Choline phosphatase 1
Protein Accession Number:
NP_002653.1
Protein Gi Number:
4505873
Ncbi Gene Id Number:
5337
Background:
Phosphatidylcholine (PC)-specific phospholipases D (PLDs; EC 3.1.4.4) catalyze the hydrolysis of PC to produce phosphatidic acid and choline. A range of agonists acting through G protein-coupled receptors and receptor tyrosine kinases stimulate this hydrolysis. PC-specific PLD activity has been implicated in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis.
Background Reference 1:
Hammond S.M., J. Biol. Chem. 272:3860-3868(1997) [PubMed: 9013646].
Background Reference 2:
Steed P.M., FASEB J. 12:1309-1317(1998) [PubMed: 9761774].
Background Reference 3:
Lopez I., J. Biol. Chem. 273:12846-12852(1998) [PubMed: 9582313].

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-927

List Size:
0.1 mg

List Price:

$376.00

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