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Antibody used in WB on Transfected 293T at 0.2-1 ug/ml.
Antibody used in WB on Mouse liver at: 1:1000 (Lane 1 - 4: 22ug mouse liver cell lysate).
Antibody used in WB on Transfected 293T at 0.2-1 ug/ml.

PLD2 Antibody

CATALOG NUMBER: 25-111

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PLD2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
PLD2 antibody can be used for detection of PLD2 by ELISA at 1:62500. PLD2 antibody can be used for detection of PLD2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Tranfected 293T Cell Lysate
Predicted Molecular Weight:
106 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store PLD2 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
PLD2
Additional Names:
PLD2,
Protein Accession Number:
NP_002654
Protein Gi Number:
156564407
Ncbi Gene Id Number:
5338
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Phosphatidylcholine (PC)-specific phospholipases D (PLDs; EC 3.1.4.4) catalyze the hydrolysis of PC to produce phosphatidic acid and choline. Activation of PC-specific PLDs occurs as a consequence of agonist stimulation of both tyrosine kinase and G protein-coupled receptors. PC-specific PLDs have been proposed to function in regulated secretion, cytoskeletal reorganization, transcriptional regulation, and cell cycle control.Phosphatidylcholine (PC)-specific phospholipases D (PLDs; EC 3.1.4.4) catalyze the hydrolysis of PC to produce phosphatidic acid and choline. Activation of PC-specific PLDs occurs as a consequence of agonist stimulation of both tyrosine kinase and G protein-coupled receptors. PC-specific PLDs have been proposed to function in regulated secretion, cytoskeletal reorganization, transcriptional regulation, and cell cycle control.
Background Reference 1:
Gorshkova, I., (2008) J. Biol. Chem. 283 (17), 11794-11806.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

25-111

List Size:
100 ul

List Price:

$519.00

Datasheet
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