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Western blot analysis of extracts from Jurkat cells treated with TNF using PLD2 (Phospho-Tyr169) Antibody. The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human brain tissue using PLD2 (Phospho-Tyr169) antibody (left) or the same antibody preincubated with blocking peptide (right).
Western blot analysis of extracts from Jurkat cells treated with TNF using PLD2 (Phospho-Tyr169) Antibody. The lane on the right is treated with the antigen-specific peptide.

PLD2 (phospho Tyr169) Antibody

CATALOG NUMBER: 79-922

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
PLD2 (phospho Tyr169) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 169 (E-N-Y (p)-L-N) derived from Human PLD2.
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Specificity:
The antibody detects endogenous levels of PLD2 only when phosphorylated at tyrosine 169.
Predicted Molecular Weight:
95 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
PLD2
Additional Names:
PLD 2, PLD1C, choline phosphatase 2
Protein Accession Number:
NP_002654.3
Protein Gi Number:
156564407
Ncbi Gene Id Number:
5338
Background:
Phosphatidylcholine (PC)-specific phospholipases D (PLDs) catalyze the hydrolysis of PC to produce phosphatidic acid and choline. Activation of PC-specific PLDs occurs as a consequence of agonist stimulation of both tyrosine kinase and G protein-coupled receptors. PC-specific PLDs have been proposed to function in regulated secretion, cytoskeletal reorganization, transcriptional regulation, and cell cycle control.
Background Reference 1:
Steed P.M., FASEB J. 12:1309-1317(1998).
Background Reference 2:
Lopez I., J. Biol. Chem. 273:12846-12852(1998).
Background Reference 3:
Divecha N., EMBO J. 19:5440-5449(2000).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-922

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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