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Western blot analysis in HL-60 cell line lysates (35ug/lane).
Western blot analysis in HL-60 cell line lysates (35ug/lane).

PLEKHF2 Antibody

CATALOG NUMBER: 58-742

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This PLEKHF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 71-98 amino acids from the Central region of human PLEKHF2.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
28 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PLEKHF2
Additional Names:
Pleckstrin homology domain-containing family F member 2, PH domain-containing family F member 2, Endoplasmic reticulum-associated apoptosis-involved protein containing PH and FYVE domains, EAPF, PH and FYVE domain-containing protein 2, Phafin-2, Phafin2, Zinc finger FYVE domain-containing protein 18, PLEKHF2, ZFYVE18
Protein Accession Number:
Q9H8W4
Protein Gi Number:
74762744
Ncbi Gene Id Number:
79666
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PLEKHF2 (Phafin 2) is a PH and FYVE domain containing protein 2. The FYVE domain and Zinc binding domain targets proteins to membrane lipids via interaction with phosphatidylinositol 3 phosphate, PI3P. The FYVE domain and Zinc binding domain present in Fab1, YOTB, Vac1, and EEA1.
Background Reference 1:
Lin, W.J., et al. Biochem. Biophys. Res. Commun. 391(1):1043-1048(2010)
Background Reference 2:
Li, C., et al. J. Mol. Med. 86(4):471-484(2008)
Background Reference 3:
Olsen, J.V., et al. Cell 127(3):635-648(2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-742

List Size:
400 ul

List Price:

$495.00

Datasheet
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