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Mouse PLGF, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Mouse VEGF R1, Mouse IgG2a Fc Tag, low endotoxin at 2 ug/mL (100 uL/well) can bind Mouse PLGF, His Tag with a linear range of 0.2-4 ng/mL (QC tested).
Mouse PLGF, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

PLGF / PGF Recombinant Protein

CATALOG NUMBER: 11-459

Tested Applications:
ELISA, WB
Specifications
source-species:
HEK293 cells
Species:
Mouse
source-species:
HEK293 cells
Recombinant Protein Sequence:
Val 19 - Pro 158
Fusion Tag:
His Tag
Tested Applications:
ELISA, WB
Application Note:
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 17.8 kDa. The protein migrates as 27-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Predicted Molecular Weight:
17.8 kDa
Purity:
>95% as determined by SDS-PAGE.
physical-state:
Lyophilized
Buffer:
100 mM HAC, pH2.5
Storage Conditions:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
Pgf
Additional Names:
PGF,PLGF,PlGF,PGFL,SHGC-10760
Protein Accession Number:
NP_032853.1
Ncbi Gene Id Number:
18654
Background:
Placental growth factor (PGF) is also known as vascular endothelial growth factor-related protein, PLGF and PlGF2, is a member of the VEGF (vascular endothelial growth factor) sub-family - a key molecule in angiogenesis and vasculogenesis, in particular during embryogenesis. The main source of PGF during pregnancy is the placental trophoblast. PGF is also expressed in many other tissues, including the villous trophoblast. PGF is actived in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. PlGF2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. Also promotes cell tumor growth.
Background Reference 1:
Maglione D., et al., 1993, Oncogene 8 (4): 925–31.
Background Reference 2:
Khalil A., et al., 2008, PLoS ONE 3 (7): e2766.
Background Reference 3:
Khurana R., et al., 2005, Circulation 111 (21): 2828–2836.
Background Reference 4:
Rolny C., et al., 2011, Cancer Cell 19:31-44.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

11-459

List Size:
0.05 mg

List Price:

$577.00

Datasheet
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