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42-818 (0.03ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
42-818 (3.75ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-818 (0.03ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

PLN Antibody

CATALOG NUMBER: 42-818

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Rat
Homology:
Expected Species Reactivity based on sequence homology: Pig, Cow
Immunogen:
The immunogen for this antibody is: KVQYLTRSAIRR-C
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 13kDa band observed in Human and Rat Heart lysates (calculated MW of 6.11kDa according to NP_002658.1). Recommended concentration: 0.01-0.03ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Heart. Recommended concentration: 3.75ug/ml.
Positive Control 1:
Cat. No. 1301 - Human Heart Tissue Lysate
Positive Control 2:
Cat. No. 1461 - Rat Heart Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PLN
Additional Names:
PLN, phospholamban, CMD1P, PLB, cardiac phospholamban
Protein Accession Number:
NP_002658.1
Protein Gi Number:
4505887
Ncbi Gene Id Number:
5350

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-818

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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