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Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).
Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).

PLOD3 Antibody

CATALOG NUMBER: 56-612

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This PLOD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 78-105 amino acids from the N-terminal region of human PLOD3.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
85 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PLOD3
Additional Names:
Procollagen-lysine,2-oxoglutarate 5-dioxygenase 3, Lysyl hydroxylase 3, LH3, PLOD3
Protein Accession Number:
O60568
Ncbi Gene Id Number:
8985
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a membrane-boundhomodimeric enzyme that is localized to the cisternae of the roughendoplasmic reticulum. The enzyme (cofactors iron and ascorbate)catalyzes the hydroxylation of lysyl residues in collagen-likepeptides. The resultant hydroxylysyl groups are attachment sitesfor carbohydrates in collagen and thus are critical for thestability of intermolecular crosslinks. Some patients withEhlers-Danlos syndrome type VIB have deficiencies in lysylhydroxylase activity.
Background Reference 1:
Wang, C., et al. J. Cell. Mol. Med. 13(3):508-521(2009)
Background Reference 2:
Salo, A.M., et al. Am. J. Hum. Genet. 83(4):495-503(2008)
Background Reference 3:
Salo, A.M., et al. J. Cell. Physiol. 207(3):644-653(2006)
Background Reference 4:
Wang, C., et al. Matrix Biol. 21(7):559-566(2002)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-612

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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