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46-203 (0.1ug/ml) staining of HEK293 (A) and NIH3T3 (B) nuclear cell lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-203 (5ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-203 (0.1ug/ml) staining of HEK293 (A) and NIH3T3 (B) nuclear cell lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.

PLRG1 Antibody

CATALOG NUMBER: 46-203

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-PVSWKPEIIKRKRF
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 60kDa band observed in nuclear lysates of cell lines HEK293 and NIH3T3 (calculated MW of 57.2kDa according to Human NP_002660.1 and 56.9kDa according to Mouse NP_058064.2). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Prostate. Recommended concentration: 5ug/ml.
Specificity:
This antibody is expected to recognise isoform 1 (NP_002660.1) and isoform 2 (NP_001188493.1).
Positive Control 1:
Cat. No. 1405 - Mouse Kidney Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PLRG1
Additional Names:
PLRG1, pleiotropic regulator 1 (PRL1 homolog, Arabidopsis), PRL1, MGC110980, pleiotropic regulator 1 (PRL1, Arabidopsis homolog), pleiotropic regulator 1 (PRL1homolog, Arabidopsis), pleiotropic regulator 1 (PRL1, Arabidopsis homolog)
Protein Accession Number:
NP_002660.1,NP_001188493.1
Protein Gi Number:
4505895
Ncbi Gene Id Number:
5356
Background Reference 1:
Ajuh P, Lamond AI Identification of peptide inhibitors of pre-mRNA splicing derived from theessential interaction domains of CDC5L and PLRG1 Nucleic Acids Res. 2003 Nov 1;31(21):6104-16

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-203

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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